Role of fractalkine /CX3CL1 and receptor/CX3CR1 in the pathophysiology of ovarian hypersti̇mulation syndrome (OHSS): An experimental study

In this study, Fractalkine and Fractalkine Receptor activity were investigated in the physiopathology of OHSS in rats with ovarian hyperstimulation syndrome (OHSS). In the study, ovarian hyperstimulation was induced in 10 randomly selected 20 22-day-old immature female Spraque Dawley rats by administering 10 IU FSH subcutaneously for four days and 30 IU human chorionic gonadotropin on the fifth day. Group 1 (n=10) 27-day-old normal rats and Group 2 (n=10) 27-day-old OHSS rats were divided into two groups. Afterwards, ovaries and blood samples of all groups were taken and rats were sacrificed. IL8, TNFα, HIF-1α, MIP-1 β, IFN- γ, Fractalkine, Fractalkine Receptor level activities were measured in serum and tissue. As a result of ELISA study performed in ovarian tissue and serum of all groups; a statistically significant increase was observed in IL8 (p=0.001), TNF-α (p=0.001), CX3CL1(p=0.001), CX3CR1(p=0.002), HIF-1α(p=0.003), MIP-1(p=0.001), IFN-(p=0.039) levels in serum in OHSS group compared to control group. When compared with the control group, a statistically significant increase was observed in IL8 (p=0.004), TNFα (p=0.001), CXCL (p=0.013), CXCLR (p=0.014), MIP-1 (p=0.001) levels in the tissue in the OHSS group, while a statistically significant decrease was observed in HIF-1α (p=0.108), IFN- (p=0.166) levels. In conclusion, many different mediators and systems are responsible for the pathophysiology of OHSS. Increases in fractalkine and fractalkine receptor levels provide a different perspective on the pathophysiology of OHSS. Key words: Rat, OHSS, CX3CL1,CX3CR1, Fractalkine, Fractalkine Receptor