Determination of chemical content of Prunus laurocerasus plant and investigation of anticancer activity properties by real-time cell analysis system

Prunus laurocerasus plant is divided into fruit and seed parts within the scope of the thesis called Determination of Chemical Content of Prunus laurocerasus Plant and Investigation of Anticancer Activity Properties by Real-Time Cell Analysis System. Two different extraction processes were carried out, which were boiled in water and extracted with ethyl acetate and maceration with chloroform:methanol [CHCl3:MeOH (1:1)]. Chemical component analyzes of the extracts were carried out by LC-MS/MS. Anticancer activity studies of these extracts against human cervical cancer cells (HeLa), human prostate cancer cells (PC3) and mouse brain tumor cells (C6) were performed with the xCelligence RTCA SP device. The main component of the extracts obtained by boiled in water and extraction with ethyl acetate was determined as chlorogenic acid. The main component of the extracts obtained by extraction with chloroform:methanol [CHCl3:MeOH (1:1)] was determined as quinic acid. It was found that the fruit and seed extracts obtained by the boiled in water and extracted with ethyl acetate method showed strong anticancer activity at high doses against to HeLa and C6 cells. In addition, the fruit portion of the Prunus laurocerasus plant was boiled in water and the extract obtained from the extraction of the aqueous with ethyl acetate had a strong anticancer effect at high-dose against PC3 cells.