Ranunculus constantinopolitanus (dc.) bitkisi üzerinde kalite kontrol çalişmalari

In this study, Ranunculus constantinpolitanus DC. which is wild-growing Ranunculus species in Turkey, has been thoroughly investigated by pharmacognostical approaches. In botanical investigations carried out on the plant, anotamical and microscobical features, which may be used in a prospective monograph about this plant drug were investigated. In this course of the quality control studies have been conducted according to European Pharmacopeia, loss in drying, total ash, acid-soluable ash and sulphated ash of drug were determinated, and the results were report as percentage values. In addition, the phtochemical screening was investigated extracts prepared from our plant. To determinated the biological activity of the drug has been studied by using different methods. The cytotoxicity of the drug was evaluated by brine shrimp and WST-1 cell proliferation lethality bioassays; the antimicrobial activity of the drug was determinated by using disc diffusion method and microdilution method; the antioxidant activity of the drug was determinated by DPPH method. Moreover, analysis of total phenolic substance and total flavonoid substances were studied.