Investigation of the protective effectiveness of H2S donor NaHS and sulfur apricot in experimental acute renal damage induced by cisplatin in rats

Aim: In this study, it was aimed to investigate the protective efficacy of H2S donor NaHS and sulphurous dried apricots in experimental acute kidney injury induced by cisplatin in rats. Material and Method: 40 Wistar Albino rats (250-350g, 3-5 months old, male) were grouped into four groups with 10 rats in each group: Control group, cisplatin (20 mg/kg) group, ciplatin+sulphurous apricot group and cisplatin+NaHS (100 μmol/kg) group. The activities of SOD, CAT, GPx and CSE enzymes and the levels of MDA, GSH and GSSG were measured manually using spectrophotometric methods. Creatinine, Urea, Na, Cl, K, Ca levels and AST and ALT levels were measured using the kit with the help of a biochemistry autoanalyzer. Blood antiplatelet activity measurement was measured using the Chrono Log Antiplatelet Analyzer 200 system with the help of ristocetin and adenosine diphosphate (ADP) kit. Histological examinations of kidney tissues were performed. Results: Statistically significant results were obtained in antioxidant enzyme activities (CAT, CSE) and MDA level in kidney tissue in the cisplatin group compared to the control group. The same situation was observed in SOD and GPx enzyme activities, but it was not found to be statistically significant. A statistically significant increase was observed in the blood serum ALT, AST, creatinine and urea levels in the cisplatin group compared to the control group (p<0.05). ADP and ristocetin activity results; While ADP activity decreased significantly in the cisplatin group compared to the control group (p<0.05), no significant result was observed in ristocetin. Histological findings support our biochemical results obtained in our study. Conclusion: When all these results were evaluated, it was concluded that the use of sulphurous apricots with known antioxidant effects and NaHS, which is an H2S donor, will have beneficial effects in terms of preventing the damage caused by cisplatin on the kidney. Keywords: Acute kidney injury, Hydrogen sulfide, Sulphurous apricot, Cisplatin