Role of heat schock protein 90 (HP90) on the effects of resveratrol on corpus cavernosum

Erectile dysfunction (ED) is accepted as a marcer for cardiovascular diseases. The low incidence of cardiovascular diseases in French, despite the fatty foods in their diet, has been attributed to resveratrol in red wine which has been consumed frequently by French. Interestingly the vasorelaxant mechanism of resveratrol and H2S shows similarity. Several studies has been just showed that both H2S and resveratrol contributes to erectile function and may be beneficial in ED. Our previous studies have shown that Hsp90 contributes to NO-mediated relaxation responses in penile tissue. Beside the interaction of Hsp90 with CSE, a H2S producing enzyme, has been shown in aorta. However, whether Hsp90 contributes to the relaxant effect of H2S in the penile tissues has not been investigated yet. Therefore, in the present study we aimed to investigate the role of Hsp90 in the relaxant effect of both H2S and resveratrol. Our study showed that both endogenous and egzogenous H2S-induced relaxations were dependent to Hsp90. Beside we found that Hsp90 contributes to the effect of resveratrol in penile tissue. Hsp90-dependnet effects were 1-Enhancement of H2S formation 2-Inhibition of ROS formation 3-Direct vasorelaxation 4- Augmentation in endogenous H2S-induced relaxations. These result suggest that the possible beneficial effect of resveratrol in ED may result from Hsp90-depended H2S formation. This study is important to show potential dual protective effect of Hsp90 in erectile dysfunction.