Rutin ve benzeri bazı bitkisel komponentlerin analjezi ilişkili nöronal elektrofizyolojik parametreler üzerine etkilerinin primer hücre kültüründe incelenmesi

Polyphenolic phytochemicals with analgesic effects are generating an area of interest in pain management. There are many studies in the literature on the effects of chlorogenic acid, routine and hesperidin on pain and related mechanisms / processes. No study has yet been carried out to examine the effects of these test substances, which are promising bioactive substances in this field, on dorsal root ganglion neurons, in other words at the peripheral level, through the current-voltage curve and action potential parameters. In this thesis, these parameters were investigated via getting recordings performed both in voltage-clamp and current-clamp mode, using each cell as an auto control for the recordings, for the concentrations at concentration of 1 µM, 10 µM and 100 µM. Results obtained provided that all the three tested chemicals have been found to affect the threshold of action potential firing and after-hyperpolarization potentials and reduce type A potassium current densities and maximum conductance of potassium, which suggest a possible beneficial effect on neuronal excitability and induction of analgesia. As for the mechanisms by which these chemicals affect these cells at the single channels, future examination at single channel level is required for more exhaustive understanding of the observed effects.