Effects of spermidine and putrescine applications on haploid plant obtention via ovule culture in sakiz and dark green squashes

In this study, the effects of spermidine and putrescine on the haploid plant obtention via ovule culture were investigated in Sakız and dark green squashes. As plant material, 3 different cultivars of Sakız and 3 different cultivars of dark green squashes were used. 5 mg/l 2,4-D added MS medium was used for embryo stimulation in ovule culture applications. To determine the effects of polyamines, spermidine (Spd) and putrescine (Put) were used with the concentrations of 40, 80 and 160 μM/l. Furthermore, spermidine and putrescine were used together with concentration of 80, 160 and 320 μM/l (1:1, v/v), respectively. The control group include only 5 mg/l 2,4-D. According to the results of the research, it was determined that ovule development and callus formations were at different levels in every varieties. Ovule development results were found to be more successful with polyamines added culture media than the control group. According to the result of callus formation; the best culture medias were only Put and Spd for Elida Fı, Put+Spd containing media for Roni Fı genotype, Spd and Put+Spd-containing medias for Shakila Fı variety, Put and Put+Spd-containing medias for Brillante Fı genotype, and only Put in Chivas Fı variety. Generally, the best callus growth rate was found in 40 μM Put containing media, but plant from callus was not obtained.