Invesigation of antioxidant properties saponine containing plants against radiation

ABSTRACTMaster ThesisINVESIGATION OF ANTIOXIDANT PROPERTIES SAPONINECONTAINING PLANTS AGAINST RADIATIONÖmer YALINKILIÇUniversity of Afyonkarahisar Kocatepe Graduate School of Natural and Applied SciencesDepartment of Chemistry, Supervisor: Asst. Prof. Dr. Hüseyin ENGİNARProtective effect of saponin containing extracts from three plant species used inTurkish traditional medicine against X-radiation (XR)-induced oxidative stress in ratwere evaluated for in lipid peroxidation (LPO) product and levels of glutathione:Aesculuc hippocastanum L. seed extract (AKE), Medicago sativa L. extract (YE) andSpinacia oleracea L. extract(IE). We assayed the effects of XR dose (5 Gy, 10 Gy) andXR+administration of plant extracts(25.0 mg/kg, 50.0 mg/kg) on serummalondialdehyde (MDA), glutathione (GSH), reduced ascorbic acid (AA), retinol andβ-caratone concentrations in rats. Only giving XR groups, the plasma MDA (p <0.001)value significantly increased but GSH (p <0.01), AA, retinol and β-caratoneconcentration in blood decreased with increasing XR doses. In the XR+extracts-treatedgroups, plasma concentrations of MDA were increased significantly with increasingradiation but their concentrations were decreased significantly with increasing extractconcentrations. Plasma concentrations of GSH, β-carotene, retinol and AA inXR+extracts-treated groups were decreased importantly with increasing XR but theirvalues increased with increasing extract concentrations. And also blood samples ofXR+extracts-treated groups compared with control group, GSH, β-carotene, retinol andAA significantly increased but only MDA values significantly decreased. The resultsshowed that all extract have enhanced the antioxidant status and have decreased theincidence of free radical-induced lipid peroxidation in blood sample of rats exposed XR.However, antioxidant effect of AKE given animal was more effective than that of YEand IE administered whole-body XR rats.2007, 64 pagesKeywords: Aesculuc hippocastanum L. seed extract, Medicago sativa L. extract,Spinacia oleracea L. extract, Lipid peroxidation, X-rays, Rat