The investigation of effect the levels of MDA in serum, oxide and reducte glutathione amounts and glutathione peroxidase activity in erythrocyte of smoke

ABSTRACT Master Thesis THE INVESTIGATION OF EFFECT THE LEVELS OF MDA IN SERUM, OXIDE AND REDUCTE GLUTATHIONE AMOUNTS AND GLUTATHIONE PEROXIDASE ACTIVITY IN ERYTHROCYTE OF SMOKE Nilgiin NURAL Firat University Graduate School of Naturaland Applied Sciences, Department of Chemistry 2005, Page: 35+VH In this study, glutathione peroxidase enzyme activity (GSH-Px) in erythrocyte of 26 smoked cigarette person and 30 nonsmoker persons taken as control group were determined by using UV-spectrofotometer. Besides oxide (GSSG) and reducte (GSH) glutathione amounts in erythrocytes and levels of malondialdehyde (MDA) being a product of lipid peroxidation in the blood serum of same persons were determined with high performance liquid chromatography (HPLC). Serum M DA level was s ignificantly h igher in smokers when compared t o that of controls (p<0.01). Moreover it was obtained that the levels GSSG and GSH-Px activity in erythrocyte of smokers were significantly higher than those of control group(p<0.05), while GSH amounts found to be unchanged in the smokers (p>0.05). Finally, it can explained that the increase seen in the antioxidant activity and in the MDA levels, with oxidative stress formation of cigarette. Keywords:MDA, GSH-Px, GSSG, GSH, Cigarette