Development of carrot genetic map based on SNP molecular markers

In the present research, we aimed SNP discovery via Next Generation DNA Sequencing Technology. In the study, 94 plants belonging to F2 were used because of the hybridization of the F1 plant, which is a cross-breeding line of the 2 carrot breeding lines. In the Illumina HiSeq 2000 system in the University of Wisconsin Biotechnology Laboratory, DNA fragments of each F2 plant produced by the GBS method using ApeKI enzyme were analyzed by STACKS computer software. The genotypes that result from the analysis were transferred to the Joinmap 4.0 program and Genetic Linkage Map was created. 13 Link Groups (LG) were created and a total of 1 464 SNP molecular markers ranging from 54 to 215 were placed on the map. The total length of the linking groups was determined as 793.4 cM. When the whole of the map is considered, the maximum distance between the molecular markers is 9.5 cM, and the average distance between each molecular marker is 0.54 cM. The second research on the carrot genome in the world for the discovery of SNP by the GBS method has been the work of this thesis. This map will contribute to QTL studies to determine important economic characteristics.