Investigation on detection of Plum pox virus (PPV) and Prunus necrotic leafspot virus (PNRSV) in stone fruit growing areas in Tekirdag

The survey studies have been performed in June and July of 2018 to identify the Plum pox virus (PPV) and Prunus necrotic ringspot virus (PNRSV), negatively affecting stone fruit production in its districts and Tekirdag province. During the survey studies a total of 158 leaf samples, including 71 leaves from center district Süleymanpaşa, 25 samples from Muratlı district, 19 samples from Çorlu district, 13 samples from Malkara district were collected. Thus, symptomatic leaf samples exhibiting mosaic, necrotic and chlorotic local spots, vein banding, and leaf deformations symptoms of sweet cherry (76), plum (35), peach (28), apricot (19) were tested for the presence of PPV and PNRSV by Double Antibody Sandwich Enzyme-Linked Immunosorbent Assay (DAS-ELISA). Moreover, infected sweet cherry leaf samples showing ring spot and vein banding symptoms were tested by Reverse Transcriptase Polymerase Chain Reaction (RT-PCR). DAS-ELISA test results showed that the infections of PPV and PNRSV, on 16 and 4 leaf samples, respectively. RT-PCR test results revealed that PPV was found in none of the sweet cherry leaf samples. As a result of this study were obtained the infection rate % 10.13 of PPV, while PNRSV had an infection rate as % of 2.53. The total infection rate in the Tekirdag province for both viruses were found % 12.66.