Investigation on detection of Grapevine fanleaf virus (GFLV) and Grapevine leaf roll associated-viruses (GLRV3-6) in grapevine growing areas in Tekirdag

The survey studies have been performed to identify Grapevine fanleaf virus (GFLV) and Grapevine ieaf roll associated-viruses (GLRV3-6), which have caused yield and quality losses in grapevine areas of Tekirdag province from July to August in 2018. During the survey studies, 256 symptomatic leaf samples, including 105 leaf samples in Süleymanpaşa district, 146 leaf samples in Şarköy district, and 5 leaf samples in Malkara district of Tekirdag were collected. Collected leaf samples exhibiting typical symptoms of virus diseases such as; reddish, yellowing, mosaic, necrose, fan leaf formation, rolling back, leaf deformation, and stunting symptoms were tested by Double Antibody Sandwich Enzyme-Linked Immunosorbent Assay (DAS-ELISA) method. Moreover, Reverse Transcriptase Polymerase Chain Reaction (RT-PCR) method was applied to identify GFLV to samples. According to DAS-ELISA test, 60 leaf samples were found infected with GLRaV-3 (23.44%), and 7 samples had positive with GFLV from a total of 256 symptomatic leaf samples. However, one sample had mixed infection with GFLV and GLRaV-3. RT-PCR showed that the infection with GFLV of 11 leaf samples (% 4.3). While the infection rate in Şarköy district was 33.56%, the infection rate in Süleymanpaşa was determined as 20.95%. Five leaf samples collected from Malkara district were not found infected with GFLV, GLRaV-3 and GLRaV-6 viruses. The total infection rate in Tekirdag was 27.73%. However, none of the tested samples have been identified as GLRaV-6.