The investigation of the effect on oxidative system of genistein in thioacetamide induced liver damage in rats

Hepatic damage is a serious of diseases progressing to cancer which includes viral infectious diseases (such as hepatitis B and C), steatosis, alcholic and non alcholic liver diseases (such an alcholic hapatitis and cirrhosis). These are many factors affecting prevalance of hepatic damage. These are demographic factors like age, ethnic origin; envariomental factors like hepatitis B and C viruses, aflatoxin, alchol, tobacco, iron deposition, endogenous and exogenous hormones; diet, schistosomiasis, viniyl chloride, arsenic cirrhosis, immune dusfunction, haemochromatosis and other hereditary metabolic diseases, host related factors such as obesity, diabetes, non alcoholic steatotis hepatic. Experimentally administered thioacetamide (TAA) is also an important hepatotoxic agent.In the current study, male Spraque Dawley rats weighing 200-250 g were used and rats were divided into 6 groups. Grup 1 (DMSO, n=7), %1.25 DMSO were injected subcutaneously per day for 12 weeks. Grup 2 (TAA, n=7), 200 mg/kg b.w. thioacetamide were injected intraperitonally twice a week for 12 weeks. Grup 3 (DDG, n=7), 0.2 mg/kg b.w. genistein were injected subcutaneously per day for 12 weeks. Grup 4 (DDG+TAA, n=7), 0.2 mg/kg b.w. genistein were injected subcutaneously per day for 12 weeks and 200 mg/kg b.w. thioacetamide were injected intraperitonally twice a week for 12 weeks. Grup 5 (YDG, n=7), 0.4 mg/kg b.w. genistein were injected subcutaneously per day for 12 weeks. Grup 6 (YDG+TAA, n=7), 0.4 mg/kg b.w. genistein were injected subcutaneously per day for 12 weeks and 200 mg/kg b.w. thioacetamide were injected intraperitonally twice a week for 12 weeks.At the termination, rats were decapitated under anastesia and blood samples and liver tissue samples were taken for biochemical and histopatological parameters respectively.AST, ALT and LDH in serum samples and MDA, SOD and NO in plazma samples were analysed whereas MDA, GSH-Px, SOD, CAT and NO analyses were conducted in tissue samples.TAA induced damage and effect of low and high dose of genistein were investigated by analysing GSH-Px, SOD and CAT activites in eritrocities.In conclusion, protective role of genistein aganist liver damage and lipid peroxidation in rats with TAA induced liver damage was observed. It was concluded that effects of genistein on the other parameters might be curative with time. Therefore comsumption of genistein might be an option in the prevention and the treatment of liver damage.Key words: Liver damage, genistein, thioacetamide.