Transmission rate, location and eradication processes of Tomato brown rugose fruit virus on seed

Reasons such as the increase in global seed trade and the facilitation of international transportation ensure the rapid spread of newly emerged viruses and the spread of diseases to clean areas. One of these viruses is Tomato brown rugose fruit virus (ToBRFV) disease, which has gained much importance especially in recent years. ToBRFV, It is a member of the Tobamovirus group, which also includes plant viruses such as TMV and ToMV. While ToBRFV is transmitted by seeds over long distances, it can spread very easily by contact between short distances. Active seed trade and the importance of the region we are in in agriculture are the reasons that make the transmission of ToBRFV by seed dangerous. This situation has brought the eradication studies against the viruses found in the seeds to the fore in the fight against ToBRFV. Within the scope of this thesis, some chemical and physical methods applied in the disinfection of seeds were examined. Of the physical methods, the virus density was found to be the least in seeds subjected to heat treatment at 72 °C for 72 hours. It was found that in the application of heat treatment and UV-C applications (254 nm), the virus loses its pathogenicity. According to the information obtained as a result of different disinfectant applications to seeds: 1% Tsunami 100, 1% Biocon A and 10% TSP have been determined as the optimal methods of combating this virus. In addition to the success of seed eradication, these methods do not adversely affect germination. These studies aimed at limiting the spread of the virus will make an important contribution to domestic and national seed production.