Determination of chromosome number and karyotype characterization in some wild type vetch (Vicia sp.) species from natural flora of thrace region

This study was conducted to determine the chromosome numbers and karyological characteristics of 7 different genotypes, V. pannonica subsp. pannonica, V. sativa subsp. nigra, V. villosa subsp. villosa, V. lutea subsp. hirta, V. hybrida L. species, which were naturally grown in Thrace Region of Turkey. The cytological characterization study was performed using Feulgen stain. In the study, idiograms of the species were drawn by karyotype analysis. As a result of the study, chromosome numbers of the species were determined as V. sativa subsp. nigra 2n=14, V. pannonica subsp. pannonica 2n=12, V. lutea subsp. hirta 2n=14, V. villosa subsp. villosa 2n=14, Vicia hybrida L. 2n=12, respectively. In the study, the karyotype formulas of the genotypes in the genus Vicia were determined; Vicia sativa subsp. nigra species as 4Sm + 3M in genotype 21-2, Vicia pannonica subsp. pannonica species as 4St + 2Sm in genotype 15-14, Vicia lutea subsp. hirta species as 7Sm in genotype 7-6, 7St in genotype 15-I-24, Vicia villosa subsp. villosa species as 6Sm + 1M in genotype 15-I-43, 7Sm in genotype 120-3, Vicia hybrida L. species 5St in genotype 22-1 as +1Sm. Among the genotypes studied, the shortest chromosome length was determined at Vicia sativa subsp. nigra genotype 21-2 with 2.391 µm, the longest chromosome was determined at Vicia lutea subsp. hirta genotype 15-I-24 with 8.459 µm. The lowest arm ratio has been encountered at Vicia villosa subsp. villosa genotype 15-I-43 with 1.500 µm and the highest at Vicia hybrida L. genotype 22-1 with 4.754 µm.