Analysis of DNA barcoding data of species belonging to the genus Trigonella L. (Fabaceae) and evaluation from a phylogenetic in point of view

The genus Trigonella L. belonging to the family Fabaceae is represented by about 135 species in the World. There are problems in the taxonomy of the genus Trigonella which has a high endemism rate in Turkey because of the wide geographical distribution and taxonomy of the genus based on morphology. DNA barcoding method is one of the essential molecular approaches in the solving of these problems, in addition to understanding the taxonomic status and phylogenetic relationships of the species. For this purpose, the regions belonging to nuclear and chloroplast DNA are commonly used for their species identification. In this study, sequence information of all existing barcode regions belonging to the genus Trigonella in the NCBI (National Centre of Biotechnology Information ) database was used for the taxonomy of the genus. ITS1-ITS2, GA3ox1 belonging to nuclear DNA and maK, rbcL, trnL-trnF, psbA-trnH belonging to chloroplast DNA from these regions were phylogenetically evaluated with the Maximum Parsimony (MP) method by using Molecular Evolutionary Genetics Analysis (MEGA). In this involve, ITS1-ITS2, matK and psbA-trnH regions belonging to the Trigonella genus were determined to give favorable results.