Genotype identification, of the parents and F4 generations in the triticum dicoccoides and triticum durum hybrids by using RAPD markers

ABSTRACT " Genotype identification in the Triticum dicoccoides and Triticum durum parents and F4 generations by using RAPD markers. " Pre. : Burcu ARSLAN Advisor: Prof. Dr. Süer YÜCE Institute : C.B.U. Institute of Natural and Applied Sciences, Master of Science Thesis. Wheat is an important element of the human diet. For this reason, in order to increase the quality and the quantity of the wheat, many breeding studies are breing carried out. Today, the various molecular markers that enables the observation of the genotype structures are in use. In this research, the wheat species (Triticum durumand Triticum dicoccoides) were used because of their economical and dietary importance and high protein content respectively. Their hybrid offsprings were analyzed by using RAPD markers. Six primers providing significant bands (OPA-02, OPA-04, OPA-06, OPB-03, OPB-04, OPB- 05 ) were selected and used in RAPD analysis to determine the genetic polymorphism and identifying the genotypes, based on preliminary results of 20 RAPD primers. The electrophoretic data were analyzed by cluster method (Jump). Genotype distances among the species were revealed by dendograms. In conclusions, it was determined that T.diccocoides formed another group different from the cluster parents and hybrid offsprings. This data forms a basis for the future breeding studies. Key word: RAPD, PCR, Triticum dicoccoides, Triticum durum VIII