Detection of citrus tristeza virus currently situation by the methods of serological and molecular in Turkey

This study was conducted at 23 districts of 6 different provinces are the most citrus growing areas of Mediterranean and Aegean region of Turkey. The samples were collected from Adana, Mersin, İçel, Antalya, İzmir and Aydın. Samples were collected form trees which showing symptoms like Dwarfing at different level, leaf yellowing, Slow or Quick Decline, Over-Growth at bud union, bark Stem Pitting and inverse pitting. For this aim 147 citrus orchards were surveyed and 105 and 171 leaves samples were collected in 2006 and 2007 respectively.All samples were tested by DAS-ELISA and results showed that totally 8 samples were CTV positive which are 6 samples from Mersin and 2 samples from İzmir. Four positive samples from different sources were inoculated to Mexican Lime but any typical symptoms have not been observed form indexing assay. However all positive and some suspected samples were analyzed by RT-PCR.As a result of this two years study, except the trees which founded as positive at two decades before, any new infection has not been determined in the region.Finally it is determined that the situation of CTV has no any significant change in Turkey during last 20-25 years.As a conclusion; due to the entry of the most harmful vector (Toxoptera citricidus ) of the disease to Europe like Spain and Portugal CTV is still remains as a big potential hazard for citiculture of Turkey.