Chromatographic and spectrometric assignment of bioactive compounds in the roots of Paeonia mascula L. (Mill.) subsp. mascula from different regions of Turkey

Paeonia mascula L. (Mill.) subsp. Mascula was collected from Osmaniye-Amanos, Afyon-Derecine, Konya-Doğanhisar and Çanakkale-Tombaklık. The antioxidant activity of methanol extracts of the root parts of the plants was investigated spectroscopically and their phytochemical contents were investigated by HPLC-DAD. The spectroscopic total phenolic content of the methanolic root extracts was determined as 1077,0 mg GAE /100g root in Çanakkale sample, which was the highest. The iron (III) reducing antioxidant activity (FRAP) of this extract was also found to be the highest at 2121,7 mg TE /100g root. On the other hand, the lowest SC50 value in terms of DPPH radical scavenging activity was 255,9 μg/mL in the Afyon sample. It was determined that the main components of methanolic root extracts determined by HPLC-DAD were paeoflorin, albiflorin, benzoylmudanpioside and 2,4,6-Trihydroxy-1-methyl benzoate derivative components.