The effect of systemic epidermal growth factor on brain tissue glycosaminoglycan and protein levels in wound healing

Would healing; is the end result of a process initiated by a regular, cellular and biochemical sequence of events leading to the renewal of tissues following a trauma.An effective would healing involves a series of highly organized events such as inflammation, re-epithelization, proliferation, matrix formation, angiogenesis and wound contraction. Various Growth factors such as Epidermal Growth Factors (EGFs), are proteins effective in wound healing. Systemic suplementation of EGF accelerates wound healing by stimulating epithelization and granulation tissue formation as well as the formation of new blood vessels. Just as Glycosaminoglycans (GAG) are effective in wound healing, Growth Factors may also mediate wound healing.The aim of this study is to investigate the changes of the protein level and GAG synthesized in the rat brain tissue due to the systemic suplementation of Epidermal Growth Factor (EGF) during the physiological process of wound healing.48 male Wistar ? Albino type rats weighing 200 ? 250 g each were used in the experiments. The rats were divided into two equal groups after dorso-lateral excision wounds were made on them. 1-In the first wounded group, the wounds were left untouched. 2- In the second wounded group EGF was administrated intraperitoneally on the 1st, 5th, 7th and 14th day following wounding of the rats. Rats from both groups were sacrificed on the 1st, 5th, 7th and 14th day and the GAG synthesized in the rat?s brain tissues as well as the protein levels, both being important in wound healing, were measured using spectrophotometric methods. The results were compared with Mann Whitney U Test.As a result, the study tried to contribute the fact that during the normal wound healing process of peripheral injuries; the brain GAG levels are not effected, systemic administration of EGF especially on the 7th day increased the secretion of brain GAG, as a result, protein synthesis increases on the 7th day when the tissue repairing is highest, hence contributing to the wound healing in the brain.