Biotechnological synthesis,characterization and evaluation of antibacterial and antiproteolytic activities of metallic nanoparticles for the treatment of wound and burn

ABSTRACT Objective: The aim of this thesis is to synthesize metalic nanoparticles which have strong antibacterial potential via nanobiotechnological methods and evaluate their inhibitory effects against frequently grown bacteria, Staphylococcus epidermidis and Staphylococcus aureus, in wound and burn area. Method: Metallic gold (Au), silver (Ag) and gold-silver (Au-Ag) nanoparticles were synthesized via Pelargonium sidoides root extract with nanobiofabrication method and their physicochemical characterizations were done with Fourier Transform Infrared Spectroscopy, Ultra Violet/Visible Spectroscopy, Transmission Electron Microscope and zetameter. Inhibitory effects of these nanoparticles against Staphylococcus epidermidis ATCC 12228 and Staphylococcus aureus ATCC 29213 were evaluated according to CLSI, 2016. Results: Gold, silver nanoparticles and gold-silver core-shell constructed nanoparticles were synthesized via bionanofabrication. Silver monometalic and gold-silver bimetalic nanoparticles were found to have strong antimicrobial effect against two most common pathogenic bacteria, Staphylococcus epidermidis and Staphylococcus aureus, in wounds and burns area. Bimetalic nanoparticles solution with the strongest antibacterial activitiy was found effective at lower application volumes against S. aureus and S. epidermidis at 0.01 M 25 μL and 0.01 M 20 µL respectively. Extracellular proteolytic activity of S. aureus and S. epidermidis were suppressed via 10µL - 20 μL application volumes respectively while proliferation were not supressed. Conclusion: As a result of this study nanoparticles of gold monometalic, silver monometalic and gold-silver core-shell bimetalic nanoparticles were shynhesized via Pelargonium sidoides root extract and their antimicrobial and antiproteolytic effects against Staphylococcus epidermidis and Staphylococcus aureus was determined for the first time. Key words: metalic nanoparticles, antibacterial, antiproteolytic, Staphylococcus, Pelargonium sidoides.