The effect of buckwheat bran and leuconostoc lactis probiotic bacteria on aflatoxin B1 (AFb1) bioaccessibility in almond milk

Aflatoxin B1 (AFB1) is a type of mycotoxin produced by the secondary metabolism of the fungi Aspergillus flavus and Aspergillus parasiticus. Mycotoxin induced possible health risks can be reduced with AFB1 detoxification. In this study, the binding capacity of Leuconostoc lactis as a probiotic and oat bran as a prebiotic in almond milk and its bioaccessibility level were investigated using an in vitro digestion model. The amount of free AFB1 in the created study groups (Positive and negative control, AFB1 + L. lactis, AFB1 + oat bran, AFB1 + L. lactis + oat bran) was determined after incubation and after digestion in the small intestine and colon using the in vitro digestion model (High Performance Liquid Chromatography). Determined by HPLC analysis. AFB1 binding level after incubation was found to be between 19.2-21.1%. The highest AFB1 binding capacity was seen in the group containing L. lactis + oat bran, and the lowest AFB1 binding capacity was seen in the group containing L. lactis, but the results were not statistically significant (p>0.05). Post-digestion AFB1 binding levels in the small intestine were found to be between 25.8-60.3%. The lowest AFB1 binding level was found in the group containing L. lactis probiotic bacteria and the highest AFB1 binding level was found in the group containing L. lactis + oat bran. The data was found to be statistically significant (p <0.05). Post-digestion AFB1 binding level in the colon was found to be between 39.6-51.9%, but the difference between groups was not statistically significant (p>0.05). Using an in vitro digestion model, the bioaccessibility of AFB1 in almond milk was found to be between 74.2-39.7% after digestion in the small intestine and 60.5-48.1% after digestion in the colon. After digestion in the small intestine, a statistically significant decrease was observed in the group containing L. lactis + oat bran compared to the positive control group (p <0.05). The lowest bioaccessibility after digestion in the colon was seen in the group containing L. lactis + oat bran, but it was not found to be statistically significant (p>0.05). In this study, the effects oat bran with Leuconostoc lactis probiotic bacteria on AFB1 binding capacity and bioaccessibility after in vitro digestion were demonstrated. Oat bran with L. lactis can be used as a reducing strategy of AFB1 bioaccessibility in almond milk.