Investigation of antiproliferative antiapoptotic and neuroprotective effects of alpha humulene in vitro co-culture model on SH-SY5Y cell line

Alpha humulene, the major active ingredient of the Humulus lupulus hops plant with its Latin name, is a monocyclic sesquiterpene consisting of three isoprene units containing three unpaired C═C double bonds. Apart from hops, cannabis is commonly found in sage, basil and ginseng plants. Alpha humulene is also known as alpha-Caryophyllene. Alpha humulene; it has many effects such as anti-inflammatory, antimicrobial, antioxidant, anticancer and local anesthetic. In this thesis, the MTT test was used to determine the concentration range of the cytotoxic effects of alpha humulene in the SH-SY5Y cell line, a bone marrow derived human neuroblastoma cell line. In addition, the neuroprotective effects of Alpha humulene in the model of neurotoxicity induced by H2O2, the IC50 value of Alpha humulene on undifferentiated and differentiated SH-SH5Y was determined 221 μg / mL and > 400 μg / mL cells using the Real-time cell analysis system (xCELLigence RTCA-DP). Apoptotic effect levels were determined by AO / PI staining method. The SH-SY5Y cell line and the LPS stimulated THP-1 cell line were incubated together to establish an in vitro co-culture model. In the in vitro co-culture model established, fluorescence measurements were taken in Cytation 3 Multi-Mode Reader to determine the immunological effects of Alpha humulene using LPS stimulation and multiple independent images were taken. The apoptotic effect caused by cellular damage caused by H2O2 alpha humulene was reduced.