Syntheses, characterization and immobilization of ß-galactosidase studies on (aminomethyl)polystyrene-anchored schiff bases and its Ni(ii) complex

Polymer-bound Schiff-base ligand (APS-2PIn-Sch) was prepared from (aminomethyl)polystyrene (APS) bound 2-phenlyindole-3-carboxaldehyde (2PIn-Ald) and its Ni(II)complex (APS-2PIn-Sch-Ni) was also synthesized. The (APS-2PIn-Sch) and its Ni(II)complex were characterized on the basis of elemental analyses and spectral analyses studies. (APS-2PIn-Sch) and (APS-2PIn-Sch-Ni) were further characterized thermally, employing thermogravimetry (TGA) and morphologically employing scanning electron microscopy (SEM). In addition, main units have been suggested with elemental analysis for these substances, and than the weight average molecular weight (Mw) has been suggested. After that, ß-Galactosidase were immobilized onto (APS), (APS?2PIn-Sch) and (APS-2PIn-Sch-Ni) complex with using the covalent binding. The influence of temperature, pH and reusability, storage capacity on the free and immobilized ß-Galactosidase were investigated. Kinetic parameters were calculated as 0.343mM/0.0259 mM.min?1, 0.681 mM/0.0716 mM.min?1, 0.0363 mM/0.0185 mM.min?1 and 0.380 mM/0.0718 mM.min?1, for Km/Vmax respectively; free, (APS), (APS?2PIn-Sch) and (APS?2PIn-Sch?Ni) bound enzymes. Storage capacity of (APS?2PIn-Sch?Ni) was found higher than other polymers.