The antimicrobial activities of probiotics Lactobacillus spp. against multidrug resistant pathogenic bacteria from diabetic infections

Growing scientific evidence supporting the positive role of probiotic microorganisms in human health; nevertheless, more study is required. In the current study, 170 samples were collected among them 100 of them were suffer from diabetes having foot infectious and 70 of them have no diabetic foot infections. The individuals were selected in both genders, aged between 33-78 from hospitals in Diyala province Iraq. After culturing the samples, the results showed that 54 isolates had apositive growth from the 70 samples for diabetic patients without diabetic foot infection as normal flora. while 70 isolates (70%) were positive growth for diabetic foot patients and 30 (30%) were negative growth. Selective media, VITEK 2 compact, biochemical testing, and microscope analysis were employed to identify K. pneumonia (%36), P. aeruginosa (%16), E. coli (%12), P. mirabilis (%4) ve S. epidermidis (%2). Enterobacteriaceae was most resistant to polymixin B and P. aeruginosa was mostly resistant to cefepime, ticarcillin-clavulanic acid and polymixin B. In this study indicated that probiotics have antibacterial activity ranging from 12 mm to 19 mm and anti-biofilm activity ranging from 29% to 50% in Diyala province in Iraq.