Micropropagation of some fruit rootstock with classical and next generation temporary immersion bioreactor system

In this study, aimed to determined the effect of Plantform temporary immersion bioreactor system, which is one of the next generation tissue culture techniques on micropropagation and rooting of different Prunus rootstocks (Myrobolan and Garnem) in in vitro conditions, comparatively with classical tissue culture techniques. In both systems, MS medium containing 1 mg/l BA was used in micropropagation experiments. In micropropagation assays, micropropagation rate (shoot/plant), plant height (cm), number of leaves (pieces), fresh weight (mg) and dry weight (mg) of genotypes were investigated. In both systems, MS medium containing 1 mg/l IBA was used in rooting experiments. Plant height (cm), root length (cm), root number (number), fresh weight (g) and dry weight (g) parameters were investigated. In addition, the general conditions of the plants were evaluated in micropropagation and rooting experiments. The rooting rate of the plants in Myrobolan and Garnem rootstocks was determined as 100%. For the two genotypes used in the thesis study, it was found that the overall appearance of the plants in the Plantform system was better than the solid culture and their development was better.