Use of some tetrakis derivatives as M1 receptor protein agonists in muscle cells

Muscarinic M1 acetylcholine receptors (mAChRs; G-protein coupled receptors); regulates many important functions in both the central and peripheral nervous systems. The M1 receptor, the muscarinic acetylcholine receptor, is widely seen in the cerebral cortex, hippocampus, and striatum of the brain. The M1 receptor has a very important role in learning and memory processes in human. Alzheimer, Parkinson, PSP, DLB and various diseases in which the functions of voluntary motor movements cannot be performed occur when there are changes in the 3-dimensional structure of this receptor protein, its deficiency, or any problem in the expression system. It is planned that the designed and synthesized tetrakis derivatives will bind to the M1 receptor protein to create an action potential and to create an effective alternative treatment for various diseases. These molecules are designed by in-silico approaches. Total of 4 molecules were designed and synthesized from 1 serie of molecules, and these molecules were characterized by NMR, IR, and UV-Vis spectroscopy. In in-vitro studies, the cytotoxic effects of molecules were first examined in the C2C12 cell line. It was stained using the indirect dyeing technique. So the specific agent used in the formation of neuromuscular junctions, alpha-bungarotoxin conjugated CFR, 594, and anti-SV2A antibody (E-8) using alexa fluor® 488, nicotinic acetylcholine receptors and synaptic vesicle 2 A proteins were stained, respectively, and neuromuscular junction junctions were demonstrated as immunofluorescence. M1 receptors are known to occur on the surfaces of SH-SY5Y cells that differentiate in the presence of retinoic acid. M1 receptors, which are members of the G protein-linked receptor family, affect the concentration of Ca+2, which is one of the building blocks of secondary communication pathways, in the cytoplasm and the consumption of ATP during their interaction with the molecules that they have an affinity for. For this reason, luminescence readings were made with Ca+2 and ATP kits in order to observe the effects of agonist molecules.