The combination effect of biosynthesized silver nanoparticles with antibiotic against clinical isolates

From July-September 2020 one hundred Clinical and food specimens, were collected from markets and patients from Baghdad Teaching Laboratories in addition to two environmental samples (soil). The bacterial isolate from clinical and environmental sample were identified by Vitek 2 System that showed out of 100 food and clinical samples 50 (68.8%) of there were Klebsiella pnieumonae isolates and the highest percentage isolates were obtained from burn samples 26 (52%). The isolates of P. aeruginosa (n=206) showed different susceptibility towards 11 antimicrobial agents used, There were about (45%) resist to Amox- clavulanate, (73.12%) to Piperacillin, (75.63%) to Aminoglycosides, (68.87%) to fluoroquinolones,(80.06%) to Ceftriaxone, (53.33%) to Cefoperazone, (13.70%) to Imipenem, (56.13%) to Nitrofurantion, (100%) to Co.trimoxazole, (98.37%) to Tetracycline, (94%) to levofloxacin and also about (76.09%) resist to Ceftazidime. An environment Klebsiella pnieumonae isolate was used for production of AgNPs. The silver nanoparticles were Characterized using; UV-Vis Spectroscopy, Atomic force microscope (AFM), Scanning electron microscope (SEM) and Fourier-transform infrared spectroscopy (FTIR) analysis. Clinical and food origin Klebsiella isolates were tested to form biofilm on glass surface; tube method or Christensen's, and the results showed that 6 clinical isolates (66.66%) were biofilm producers, while among eighteen food origin isolates; 10 isolates (52.38%) produced biofilm adhered to glass surface. A foodborne Klebsiella isolate were screened for production of AgNPs using their extracellular (cell free supernatant) of bacterial cultures in PH 6 and 37ºC. This alteration in color could be noticed by nicked eye. As the color intensity increased, the accumulation of AgNPs increased.