The use of rt-pcr, elisa and hematological methods in the diagnosis of canine parvovirus infection and investigation of c-reactive protein levels

In this study, real time polymerase chain reaction (RT-PCR) and ELISA methods were used in the diagnosis of Canine parvovirüs (CPV) infection and hematological and serum C-reactive protein (CRP) levels were investigated. CPV causes serious infections and fatal systemic disorders in dogs. Disease agent Canine parvovirüs (CPV) is a small, non-enveloped and single-stranded virüs within the Parvoviridae family. Since this date two types of canine parvovirüs (CPV) isolated and known as canine parvovirüs type-1 (CPV-1) and canine parvovirüs type-2 (CPV-2). CPV-2 has 3 variants known as CPV-2a, CPV-2b and CPV-2c. In this study, blood, serum and fecal samples collected from non-vaccinated 52 dogs that were brought to Adana and Osmaniye Municipalities' animal shelters and private veterinary clinics, which had complaints of lethargy, vomiting, fever and diarrhea, were collected and analyzed. While 27 of the samples examined for anti-CPV IgM and IgG antibody (CPV Ab IgM and IgG) by ELISA method were found to be positive for CPV Ab IgM and IgG (51,9%) and 29 (55,8%) were found to be positive with RT-PCR. Hematological tests were done by a veterinary autoanalyzer and serum CRP by ELISA method. The results obtained were found to contain important data in the diagnosis of CPV infection. Hematological data and serum CRP values were found to contain valuble data in the diagnosis of the disease. It was determined that there are no big differences between ELISA and RT-PCR results and 2 dogs showing clinical signs of CPV were serologically negative by ELISA, but were positive by RT-PCR test. It was concluded that the CPV Ab ELISA method in the diagnosis of CPV infection is an easily accessible, safe, practical and economical test, and RT-PCR is a fast and reliable test and should be used for the confirmatory diagnosis of serological results. Keywords: Canine parvovirüs, dog, RT-PCR, ELISA, IgM, IgG, CRP, hematology.