Çeltik kavuzundan biyohidrojen üretimi

Biohydrogen production from agro-industrial wastes such as rice husk, as a promising approach is a renewable and a clean transportable fuel process producing no greenhouse gases. In this study, Clostridium termitidis and Clostridium intestinale were evaluated under mesophilic conditions to improve biohydrogen production in co-culture and consolidated fermentation. First the cultures were tested alone for their biohydrogen production performences. Then, the experiments were carried out to determine the best co-culture ratio (1:1-20:1), rice husk particle size (<2000µm, <300 µm, <74 µm) and substrate concentration (1-20 g L-1) enhancing biohydrogen production yield and rate. The highest cumulative hydrogen formation by C. intestinale was obtained from 10 g L-1 thermal treated rice husk (14 mL), and by C. termitidis from 5 g L-1 ground rice husk (14.6 mL) where thermal treatment was not applied. Significant amounts of hydrogen production yields and rates were obtained with pure cultures. In raw rice husk the highest CHF was obtained with the co-culture ratio of 5:1 (29.15 mL). The highest hydrogen production yield with raw rice husk was obtained with the co-culture ratio of 5: 1 (38.86 mL H2 g-1 substrate) and the highest HPR was obtained with a co-culture ratio of 1:1. The highest substrate concentration gave the best cumulative volume of hydrogen (41.1 mL). The highest hydrogen production yield (90 mL H2 g-1 substrate) and hydrogen production rate (0.29 mL H2 g-1 substrate h-1) were obtained with 1 g L-1 rice husk.