Enrichment of phenolic acids by aqueous two-phase system extraction method before chromatographic determination in various food samples

In the study, 1-butyl-3-methylimidazolium tetrachloroferrate, 1-benzyl-3-methylimidazolium tetrachloroferrate, 1-allyl-3-methylimidazolium tetrachloroferrate and polyethylene glycol bis (methylimidazolium) dithetrachloroferrate ionic liquids have been synthesized. These ionic liquids have been used for the aqueous two-phase extraction of phenolic acids prior to analysis. In the study, two different aqueous two-phase extraction methods have been optimized. To compare the extraction efficiency of ionic liquids containing cationic group butyl, allyl and benzyl, a separate method has been developed with these ionic liquids and the separate method has been developed with this ionic liquid to examine the effect of polyethylene glycol bis (methylimidazolium) ditetrachloroferrate on two aqueous phases with polymer/salt system. The parameters required for the highest extraction efficiency have been optimized with both methods. Analytical parameters for phenolic acids (linear range, limit of detection, limit of determination, relative standard deviation of intraday and inter day) have been determined using two methods. The developed methods have been successfully applied to apple, pear, banana and grape samples. To show the accuracy of the developed methods with two methods, high recovery values have been obtained for the standard phenolic acid concentrations added to the real samples.