Detection of genetic diversity in çine çaparı sheep by RAPD technique

SUMMARYThe aim of this study was to determine within breed genetic diversity inendangered Çine Çaparı sheep by Random Amplified Polymorphic DNA (RAPD)markers and to obtain genetic similarities and distances among animals within flock andalso between flocks. Seventy two animals from 3 flocks (26 animals from ADU-ÇÇKPconservation flock, 32 animals from Erdoğan Aktürk?s flock (EA) in Tatarmemişlervillage and 14 animals from Mustafa Vural?s flock (MV) from Dereköy village) weregenotyped with 24 arbitrary primers. Genetic similarities between and within flockswere investigated.DNA was extracted from blood. DNA amplification was realized by PCR using10?mer RAPD primers. PCR products was separated on agarose gels and photographedafter ethidium bromide staining. Band sizes and profiles were obtained from gelphotographs and a data matrix including band profiles were generated. Data wereanalyzed by using the formula of Nei for genetic similarities and distances.The genetic similarities between individuals within a flock are ranged between0.4468 and 0.8511 for ADÜ-ÇÇKP conservation flock, 0.4894 and 0.8723 for EA flock,and 0.5745 and 0.8723 for MV flock. Genetic distances were ranged between 0.1613 ile0.8056 for ADÜ-ÇÇKP flock, 0.1366 and 0.7147 for the EA flock, and 0.1366 and0.5543 for the MV flock. The similarities between flocks were ranged between 0.8439and 0.9037 and, genetic distances were ranged between 0.0902 and 0.1698.As a result, the similarity was highest between ADU-ÇÇKP conservation flockand the flock in Tatarmemişler than the flock in Dereköy village. This may be stem fromhigher animal flow between ADÜ-ÇÇKP conservation flock and flock in Tatarmemişlervillage. Mean genetic similarity between animals within flock were found highest forMV flock. This finding was also supported by fixation of frequency of nearly one-thirdof the loci in MV flock.