Decidual ve plazma kompleman düzenleyici genlerin ekspresyon düzeyleri ile maternal serum anti-trophoblastik antikor (ATA) düzeylerinin idiyopatik tekrarlayan ve spontan abortlarda araştirilmasi

Objective Recurrent pregnancy loss is defined as the loss of two or more pregnancies before the twentieth gestational week. This complication cannot be fully explained in some cases due to its multifactorial pathogenesis. In this study, it was aimed to investigate complement gene expression and C3a, C5a complement products effective in pathogenesis. In addition, it was planned to examine anti-HLA and ATA serum factors for the detection of antibody-mediated reaction development. Materials and methods Those with spontaneous abortion (n = 9) were used as the control group, and those with recurrent pregnancy loss (n = 16) were used as the patient group. Tissue CD55, CD46 and CD59 expression levels were studied with the simultaneous PCR method. C3a, C5a and anti-trophoblastic antibodies, which are important for fetal survival, were analyzed by ELISA method. Results In our study, CD55 expression level was higher and serum C3a level was lower in the patient group compared to the control group (p <0.05). Although CD46 expression levels were different between the two groups, it was not as remarkable as the increase in CD55 expression (p> 0.05). No sensitization reaction in terms of HLA was detected against the spouses of the analyzed patients. Conclusion In conclusion, the high CD55 expression observed in the case group in our study compared to controls indicates complement-mediated tissue damage. It can be thought that complement system regulators, especially CD55 and C3a, can be a potential biomarker in order to predict recurrent miscarriages before they occur and to prevent them with early and appropriate treatment. Key words Recurrent pregnancy loss, decidual CD59/CD55/CD46 expression, complement activation