The effect of different doses on the efficiency of LLLT in midpalatal suture using histomorphometry

We aimed to investigate the effect of different doses 940 nm wavelength laser (InGaAsP) applied after midpalatal expansion in rats on sutural activity histomorphometrically at cellular level in rats. In our study, male Wistar rats weighing 180-220 grams were used for 80-12 weeks. The rats were irradiated with Biolase Epic Indium Gallium Arsenide Phosphor (InGaAsP) Diode Laser (wavelength 940 ± 10 nm, power output 0.1 W, continuous mode, frequency 50/60 Hz). The groups were formed as control group, low dose laser group (18 J), medium dose laser group (42 J), high dose laser group (60 J). On day 7, half of the groups in each group were sacrificed and the same laser application procedure as in the remaining rats was continued until the end of day 21. The expansion appliance was applied with a force of 70 grams lateral to the maxillary incisiors of the rats. Histological evaluation revealed that laser low-dose laser group (18 J) after maxillary expansion had a biostimulatory role of laser on bone healing compared to other groups. As a result of statistical evaluation, osteoblast, osteocyte, new bone formation values of low dose laser group were found to increase. According to these results, it might be possible that low-level laser application can shorten the duration of retantion treatment, which is one of the biggest problems in orthodontics.