The effect of n-acetyl cystein and methyl-prednisolone on acute pancreatitis in an experimental model

The study was performed Surgical approaches were performed in operation room of fizyololoji deparment and financed by Science Research and Project improvement deparment of Cukurova University. The purpose of this study was to examine the effect of N-asetilsistein and metal-prednisolon on cerulein experimental pankreatitis model by using short and long term results of amylase, lipase and histopathologic scores of pancreatitis and pulmonary complication. The study was conducted with 64 Wistar-Albino adult rats weighing 175 to 240 gr and divided in to four groups which were divided in to two equal groups. Subcutan saline was injected to the rats in Group 1 for control group. Subcutan cerulein was injected to the rats in Group 2 for experimental pancreatitis group. In group 3, rats were injected with intraperitoneal N-asetilsistein and subcutan cerulein and in group 4, rats were injected with ıntramuscular metilprednisolon with subcutan cerulein. Group 3 and 4 were accepted as two different treatment groups. Decapitation was performed 7 hours after injection to the first half of the groups and 24 hours after injection to the second half of the groups. The amylase and lipase values, pancreatitis and pulmonary complication scores at seventh hour were accepted as short-term results. The amylase and lipase values, pancreatitis and pulmonary complication scores at twenty fourth hour were accepted as long-term results. The histopathological evalutions were done by pathology specialist not informed about evaluated groups. The edema, vacuolization, inflammation and necrosis of pancreas were scored with scoring system varying 1 to 4 (schönberg). The edema, inflammation and peribronchitis of lung were scored with scoring system varying 1 to 3. For the presentation of experimental pancreatitis occurrence, the short and long term results of amylase, lipase and histopathologic scores of pancreas and pulmoner complication of control group compared with the results of other groups (group2,3 and 4). The specimens of panceas and lung of groups which were suffered from pancreatitis were underwent immune-histochemical study with myeloperoxidase and IL-8 paints for the presentation of neutrophile infiltration. Additionally, the correlation between pulmoner complication scores and painting of lung tissues with iNOS was examined. Due to possible increase of wet weight of lung secondary to edema and inflammation, the ratio of wet/dry lung was analysed. When the short term results of amylase, lipase and histopathologic scores of pancreas and pulmoner complication of control group compared with the results of other groups, the differences exhibited statistical significance. On the other hand, when the long term results compared only pancreatitis group (Grup 2) demonstrated statistical significance. 1 When the histopathological results of lung specimens of control group compared with the other groups, Group 2,3 and 4 have significant hemorrhagic areas, edema and peribronchial mononuclear cell infiltration. Neutrophile infiltration was demonstrated with myeloperoxidase and IL-8 painting in histopatologic specimens of lung and pancreas of Group2, 3 and 4. The direct correlation was established between histopathologic scores of pulmonary complication and iNOS painting. Dry/wet ratio of lung tissues was higher in group 2, 3 and 4, however no statistical significance was established. When the short- term results of amylase, lipase and histopathologic scores of pancreas and pulmoner complication of Group 2 (pancreatitis group) compared with the results of other treatment groups ( Group 3 and 4), the differences between short- term results exhibited statistical significance. In the comparison of long-term results of amylase, lipase and histopathologic scores of pancreas and pulmonary complication between group 2 and 3, 4; group 4 (metal prednisolon) demonstrated statistical significance with respect to all parameters, but Group 3 ( N-asetilsistein) exhibited statistical significance with respect to amylase, lipase and histopathologic pancreatitis scores. Hence, Group 3 had no statistical difference according to pulmonary complication scores. Conclusion: In this study, it was established that cerulein is an appropriate agent for experimental pancreatitis model and N-asetilsistein and metal-prednisolon had a therapeutic effect on experimental pancreatitis. Additionally, in short term metilprednisolon and N- asetilsistein had an effect on pulmonary complication, however according to our findings in long term N-asetilsistein had no potential for pulmonary complication.