Comparison of some acute phase protein levels in rats with experimentally induced infectious and noninfectious inflammation

Acute phase reaction is a nonspesific reaction that occurs infectious, immunulogic,neoplastic, traumatic or parasitic causes. Most important features of acute phase reaction isthe production of some acute phase proteins such as CRP, SAA, haptoglobin,ceruloplasmin, fibrinogen in liver.In this study, it was investigated that the levels of acute phase proteins in rats withexperimentally induced different inflammation models. In first groups rats were treatedwith 0,5 mg/kg turbentin oil (single dose, ip) for noninfectious inflammation. Forinfectious inflammation model, 21 rats that were in second group were treated by 106 CFUStaph. auresus (ip). 21 rats in control group were given FTS in same way. Additionally,blood samples were collected to 7 healty animals for 0 days. Days of 1., 4. ve 7., bloodsamples were collected intracardiac way in randomly selected 7 rats under ether anesthesiaand applied othenasia by cervical dislocation. Whole blood samples were used forfibrinogen analyses and blood counting; serum samples were analysed CRP, SAA,haptoglobin in ELISA reader, ceruloplasmin, total protein and albumin analyses weremeasuring by spectrophotometric methods. Serum protein fractions were determined withelectrophoresis.As a result of this study, haptoglobin, fibrinogen and ceruloplasmin levels weresignificantly increased in rats with acute infectious and noninfectious inflammation groupwhen compare between blood samplind days and in day before induction. CRP and SAAlevels were increased but were not determined any statistically significance. There werealso observed that haptoglobin and fibrinogen concentrations were higher in infectious.Key Words; Inflammation, infectious, noninfectious, acute phase proteins