Use of soluble complement receptor type 1 to prevent local and distant organ injury in a rat intestinal ischemia reperfusion model

Introduction: In this experimental study we aimed to examine in vivo effect of soluble complement receptor type1 (SCR1) in preventing local and distant organ injury in ischemia reperfusion model via superior mesenteric artery (SMA). With these data, it would be possible to have idea about the clinical usage of SCR1. Material and Methods: 24 male rats, weighing between 200-250gr, are classified into four groups. In group 1; SMA was clamped for 60 minutes. In group 2; after laparatomy intravenous (IV) SCR1 was given. In group 3; SMA was clamped for 60 minutes, after ischemia IV SCR1 was given at the 60th minute, 1 minute later reperfusion was done. Group 4 was the laparatomy group. To investigate the organ injury, liver function tests (serum AST and ALT levels) and kidney function tests (serum BUN and creatinin levels) were done. To evaluate the systemic and local effects of inflammation total serum levels of protein, albumin, tumor necrosis factor- alpha (TNF-α) and interleukin-6 (IL-6) were tested.In tissue samples glutathione (GSH), malondialdehid (MDA) and myeloperoxidase (MPO) positive neutrophil counts were identified. Results: According to the statistical analysis SCR1 was shown to reduce the ischemia-reperfusion injury and have anti inflammatory effects. It was pointed that distant organ injury due to reperfusion was prevented by SCR1. Conclusion: SCR1 was verified to decrease the mortality and morbidity