Levels of interleukin 6 and tumor necrosis factor alfa in serum from fibrosarcoma induced rats

Cytokines, produced by virtually all types of cells, are multifunctional polypeptide. TNF-alfa is a plethoric cytokine that has a major role in natural and acquired immune, cell regulation and differentiation and apoptosis. IL-6 has a lot of functions in natural and acquired immune, inhibit cell growth at carcinoma and leukemia without B cell, tissue?s growthing and differential. It is very important at the prognosis, survival, available therapy in cancer. In this study, serum IL-6 and TNF-alfa ? levels were investigated in fibrosarcoma induced by 3-methylcholantrene.Sixteen male Spraque Dawley rats were used in this study. They were 8 weeks old and the body weights of the rats were between 150-200 grams. Rats were allocated into two groups each containing 8 rats. During the experiment which took between 150-210 days depending on the apperance of tumor tissue. They were allowed free access to water and feed in order to induce fibrosarcoma in rats, at the beginning of the experiment animals were injected subcutaneously on the neck with 0,2 mg 3- methylcholantrene solved in 0,25 ml sesame oil. To find out weather any tumor tissue occurred animals were palpated daily. At the end of the experiment, the blood of animals was taken from atrium of the hearth under the ether anesthesia and than serum was prepared. Serum IL-6 and TNF-alfa ?? levels were analyzed using BioSource kits at ELISA.Serum IL-6 levels were found 67, 85 ± 3,13 pg/ml in experimental group, 38, 45 ± 3,39 pg/ml in control group. It was 1, 8 times higher than controls. Serum TNF-alfa ? levels were 53, 35 ± 9,08 pg/ml in experimental group, 26, 42 ± 2,22 pg/ml in control group. It was 2 times higher than controls.In the light of this finding ıt is thought that results of study are beneficial to understand pathogenesis and prognosis of cancer.Key words; Fibrocarcoma, IL-6, TNF-alfa ?