Fırst six hours of serum pNF-H levels in rats with experimental mild head trauma

Fırst Six Hours of Serum pNF-H Levels in Rats with Experimental Mild Head Trauma The aim of our study is to investigate the course of Phosphorylated Neurofilament Heavy Chain, which can be used as a biomarker to define traumatic brain injury, in the first six hours after trauma in serum samples taken from rats with experimentally mild traumatic brain injury. In our study, which was designed as an animal experiment, 32 female Spraque-Dawley rats were used. The subjects were enrolled equally (n=8) into 4 groups as 3 experimental groups and 1 control group. Traumatic brain injury was created with a 50 gr ball dropped to the subjects by free fall from a height of 80 cm. Serum Phosphorylated Neurofilament Heavy Chain level was measured without creating traumatic brain injury in the control group, and at the 2nd, 4th and 6th hours after traumatic brain injury in the experimental groups. Serum Phosphorylated Neurofilament Heavy Chain values measured at the 2nd and 4th hours after traumatic brain injury were statistically significantly higher than the control group. Serum Phosphorylated Neurofilament Heavy Chain levels gradually decreased at the 4th and 6th hours compared to the 2nd hour, and dropped to similar levels with the control group at the 6th hour. Briefly, it was thought that a high serum Phosphorylated Neurofilament Heavy Chain value, especially in the first 2 or at most in the first 4 hours, could be used in the diagnosis of traumatic brain injury in patients who developed mild traumatic brain injury after trauma and could not be detected by imaging Keywords: Emergency Room, Head Trauma, Mild Traumatic Brain Injury, Phosphorylated Neurofilament Heavy Chain