Introduction and Objective: The aim of this experimental study is to research the effects of L-alanin and L-glutamine on liver, structural changes on small intestine, wound healing, and bacterial translocation which are being widely used on intensive care patients.

SUMMARY Introduction and Objective: The aim of this experimental study is to research the effects of L-alanin and L-glutamine on liver, structural changes on small intestine, wound healing, and bacterial translocation which are being widely used on intensive care patients. Material and Method: 20 male Sprague Dawley rats which weigh 190-230 gr used in this study. Rats separated into two randomized groups. In accordance with experimental rat burn model, skin burn was created in every rat and both groups are fed equally for 21 days. Experimental group administered L-glutamine+L-alanin containing product (Dipeptiven®, Fresenius Kabi BadHamborg, Germany) for 5 ml/kg/day orally. At the end of 21st day all rats were sacrificed. Biopsy materials obtained from liver, small intestine, and burn wound and blood culture was taken under sterile conditions. In pathologic examination structural changes in tissues and wound healing was evaluated. In microbiologic examination, effects of L-glutamine and L-alanin on bacterial translocation are evaluated by comparing small instestine and blood cultures. Results: No structural difference was seen among small intestine biopsies between two groups. No liver damage was seen in experimental group while live biopsies showed grade 1 damage in control group. Epitelization and granulation was detected as better in pathologic examination of skin biopsies obtained from burn wound showed better in experimental group. Microbiologic examination showed bacterial replication in total of 4 rats from control group, two rats showed replication of S.aures in small intestine and blood culture and two rats showed replication of S. epidermidis in small intestine and blood culture. No replication was determined in any rats from experimental group. Conclusion: In the light of findings acquired from this study done with experimental rat burn model, we think that use of L-glutamine+L-alanin (Dipeptiven®, Fresenius Kabi BadHamborg, Germany) will have positive effects on wound healing and helps preventing the bacterial translocation. We suggest regular use of L-glutamine+L-alanine in nutrition of hospitalized burn patients as a supporting product.