Determination of genetic variability and distance between numbers of Candida spp isolated from urinary tract infections in the Iraqi female population using RAPD molecular techniques

Background: Candida is a yeast that is normally found on the skin, mouth, digestive tract, and vagina. It usually causes no harm, but under certain conditions, Candida can grow excessively on the mucous membrane and moist areas of the skin, typical areas of infection are the mouth, the upper thigh, the armpits, the spaces between the fingers and toes, on the uncircumcised penis, the skin folds under the breast, the nails, and the skin folds of the abdomen.Methodology 100 samples were isolated from people suffering from urinary tract infections using sterile swap s and isolated on their selective media Dry heat sterilization oven was used to sterilize glassware at 160-1800C for 2-3 hours while moist thermal sterilization was employed to disinfect the media and certain solutions (not impacted by heating). All clinical samples of suspected lesions were infected with chloramphenicol on SDA Cycloheximide supplementation intermediate using a sterile inoculation ring and incubated at 25-30°C before being assessed. Negative cultures were after 4 weeks, whereas positive cultures were inspected by macroscopic and microscopic identification All clinical samples of suspected lesions were infected with chloramphenicol on SDA Cycloheximide supplementation intermediate using a sterile inoculation ring and incubated at 25-30°C before being assessed.