Investigation of possible changes in serum adiponectin, adipsin and visfatin levels of diabetic rats

Aim: Diyabetes Mellitus (DM); is a chronic metabolic disease characterized by relative or absolute insufficiency of insulin secretion or insulin resistance resulting in impaired carbohydrate, lipid and protein metabolism. Diyabetes is one of the major causes of organ failure and death with its complications. In addition, the cost of treatment is a highly health problem. In recent years, many bioactive molecules, expressed as adipokines, have been shown to exacerbate diyabetes, others have antidiabetic effects and reduce diabetic complications. When the existing literature information is taken into consideration; the role of adipokines known as adiponectin, adipsin and visfatin in the emergence of diyabetes and the possible relationships between each other are not clear enough. For this reason, in our experimental research project; the serum levels of the adipokines will be examined in diabetic rats and the effects of possible changes on glucose metabolism will be investigated. In addition, the association of adipokines referred in this study will be determined. Material and Method: 21 Wistar albino male rats weighing 380-434 g were obtained from Dicle University Experimental Animal Research Unit. During the experiment, these rats were found in a 12 hour light 12 hours of light rhythm in the lighted environment and the temperature of the room was adjusted to 22 ± 2 ° C basal diet was provided in rooms with a humidity of 55%.21 adult male Wistar Albino rats were divided into 3 groups with 7 each. Group I (7 rats in the healthy control group) were determined as Group II (7 rats in the diabetic control group) and Group II (Treatment (Metformin) group 7). Adiponectin, visfatin and adipsin levels of the groups were recorded by taking their blood. SPSS 21.0 program was used to determine whether there was a statistically significant difference between the groups. Results: The body weights of diabetic group rats were significantly lower and statistically significant (p <0.001). In addition, blood glucose levels in both diabetic and metformin group rats were significantly higher and statistically significant (p <0.001). Fasting insulin values were found as control, diabetic and metformin group) respectively, (1.43 ± 0.11, 0.74 ± 0.12, 1.07 ± 0.13). There was a statistically significant difference between the groups (p <0.05). Adiponectin and adipsin levels were lower in diabetic group compared to both control and metformin groups and statistically significant (p<0.05). In addition, visfatin levels were higher in diabetic group compared to both control and metformin groups and statistically significant (p <0.05). Conclusion: Glucose levels and visfatin levels were higher in diabetic rats compared to the control and metformin groups, whereas adiponectin, adipsin and insulin resistance values were lower.