Determination of antibacterial activities of new type chemical formulations against some tomato disase agents

Pseudomonas syringae pv. tomato (Pst), Xanthomonas euvesicatoria (Xeu) isolates, which are important bacterial disease factors in tomato cultivation, cause bacterial blemish and bacterial spots on the plant, causing product quality deterioration and serious losses in yield.There are no effective, licensed antibacterial products that can be used to combat these disease agents in tomato plants.In this study; The creation of new types of formulas which can provide antimicrobial effect on Pst and Xeu isolates, which are important disease agents in Tomato (Solanum lycopersicum L.) agricultural areas, and the antimicrobial activities of these formulas on target microorganisms were investigated. The effective doses of the selected actives for this purpose were determined by the Minimum Inhibition Concentration Method (MIC) under in vitro conditions. Target microorganisms were identified by MALDI-TOF MS. Formulations were formed with the actives whose activities were determined by the (MIC) Method under in vitro conditions. The efficacy of the formulations was tested by Disk Diffusion Method (DDM) on Pst and Xeu isolates under in vitro conditions. In triplicate each formulation was compared with the antibiotic disk used as a positive control. The efficacy of the formulations was calculated by measuring the clean areas they created in petri dishes. As a result of this study, it was determined that all 10 different formulations were effective on, Pst and Xeu isolates (8-30 mm). Considering the test results obtained, it was determined that these formulas could be used in chemical control against target microorganisms. In order for these formulations to be used in agricultural areas, in vivo trials should be conducted and their degradation in soil and plant tissue should be examined.