Detecti̇ng posi̇ti̇ve surgi̇cal margi̇ns of prostate tumor ti̇ssue by usi̇ng elasti̇c li̇ght scatteri̇ng spectroscopy system

Elastic light scattering spectroscopy (ELSS) system with a single optical fiber probe was employed to detect positive surgical margins of prostate cancer in combination with principal components analysis and linear discriminant analysis. ELSSS spectra in the 450-750 nm wavelength regions were obtained from the total of 299 tissue samples of 27 patients. The ELSSS spectral data were compared against the gold standard histopathology results. Data analysis was done using principal components analysis, followed by linear discriminant analysis. Receiver operating characteristic curve analysis was employed for differentiating performance. While the classification based on discriminant score provided sensitivity of 100% and specificity of 95.2%, in differentiating benign from malign surgical margins of prostate tissue and the area under the receiver operating characteristic curve 0.98, respectively. ELSSS system can accurately distinguish benign and malign surgical margins of prostate tissues with high sensitivity and specificity. Diagnostic accuracies show in this study the general viability of in-vivo fiber optical tissue differentiation.