Elucidating the biochemical activity and phytochemical content of anthemis tricornis eig species

Aim: The genus Anthemis, particularly A. tricornis, is significant in traditional medicine, especially in Turkey, due to its diverse therapeutic properties. This master's thesis focuses on the biochemical activity of A. tricornis extract, emphasizing the role of phytochemicals, specifically flavonoids and phenolic acids, in its medicinal efficacy. Materials and Methods: The study assessed antioxidant activities using various methods. Free radical scavenging methods included DPPH•, ABTS•+, and DMPD•+, which evaluate the plant's ability to combat oxidative stress. Additionally, reducing capacity was measured through methods like CUPRAC, FRAP, and Fe3+. The quantification of total phenolic acids and flavonoids was performed using LC-MS/MS technology for accurate chemical profiling. Results: In this research, the chemical compositions and biological activities of extract obtained from A. tricornis was studied. The amounts of total phenolic acid and flavonoid components in ethanol extract was determined by expression as gallic acid and quercetin equivalents, respectively (57.88 μg GAE/mg), (41.16 μg QE/mg). The extract obtained from A. tricornis showed strong antioxidant activity, along with the ability to inhibit enzymes such as carbonic anhydrase II (CA II), acetylcholinesterase (AChE), and α-amylase (α-Alz) , thereby enhancing the therapeutic potential of this plant. Conclusion: In conclusion, the thesis demonstrates that A. tricornis is rich in secondary metabolites, exhibiting notable biological activities related to antioxidant capacity and enzyme inhibition. This research serves as a foundation for further exploration of the psychopharmacological properties of A. tricornis, potentially aiding in the development of new therapies in both traditional and modern medicine. Keywords: Secondary metabolites, A. tricornis, antioxidants, enzyme inhibitors, LC-MS/MS.