Implantation potential in organoid from mouse endometrium

In this study, we were aimed to create an endometrial organoid from mice, to examine the implantation potential of blastocysts in the formed endometrial organoid, and to examine the role of human-derived mesenchymal stem cells in both organoid formation and implantation success.For this purpose, 6-8 weeks old Balb-c female mice in estrus period were used. Simultaneously, on embryonic 3,5. blastocysts collected daily were added according to study groups and cultured.Study groups; endometrial organoid culture in proliferation stage (group 1), 48 hours indirect co-culture with proliferation stage endometrial organoid iMKH (group 2), endometrial organoid culture in secretion stage (group 3a), endometrial organoid blascocyte culture in secretion stage (group 3b), endometrial organoid iMKH was created as 48 hours indirect co-culture (group 4a) and secretory endometrial organoid iMKH 48 hours indirect co-culture blastocyst culture (group 4b). After the study, the distribution of LIFR, pan-cytokeratin, mucin 1, MMP9 and CD146 for immunohistochemical analysis with hematoxylin-eosin staining for model histochemical analysis was evaluated using image j analysis method. According to the study groups, gland and epithelial structures were clearly seen in Hematoxylin-Eosin staining. In indirect immunohistochemistry staining, protein immunoreactivities examined according to study groups were found to be different, reflecting normal endometrial tissue. It was concluded that the in vitro endometrial organoid model could be created due to the significant differences between the protocol and the factors used in the study and the proliferation-secretory period, the groups in which IMKH and blastocyst were added.