Efficacy of stem cell threapy in different experimental prematür ovarian insufficiency models

Aim: In the Premature Ovarian Insufficiency (POI) induced by cyclophosphamide(CYP) and 4-Vinylcyclodiepoxide hexene(VCD) in rats, it is aimed to show the effects of Adipose Tissue-Derived Mesenchymal Stem Cells(ADMSC) and the NISCHE on some molecules that are thought to be effective in POI. Materials and Methods: Eight weeks old Wistar Albino rats were divided into 8 groups. POI was induced by administering 2 doses of 120mg/kg CYP to 21 rats at 1week interval, and 240mg/kg VCD to 21 rats for 5 days. ADMSC injection was performed as i.p for 3 days consecutively 1x106 cells. As for the NICHE 0,1 injection was performed as i.p for 3 consecutive days. H&E and Masson Trichrome staining were done. Serum AMH level was measured by ELISA. iNOS, Casp3, Cx43, Panx1 levels were analyzed by immunohistochemistry, qPCR methods. Results: In both POI models, different amounts of degenerations were observed. In POI groups, iNOS, Casp3, Panx1 expressions increased while Cx43 expression decreased. In ADMSC and NISCHE groups, expressions of these molecules were found close to control. Conclusions: Both VCD and CYP were identified as suitable agents to create an experimental POI model. While VCD mostly caused degenerations in primordial and primary follicles, CYP mostly affected developing follicles. It is thought that the molecules mentioned are important in the formation of POI and these molecules will be effective in illuminating the mechanism of the disease. The healing effects of YDMKH and NİŞ show that it will contribute to the development of new methods for the treatment of POI.