Evaluation of sperm cryopreservation and its results with different cryoprotectant solutions

Cells and tissues are cooled at very low temperature, all biological activities to stop, minimal damage and function without loss of long-term use the purpose of future storage. We investigated the effect of cryoprotectant on DNA fragmentation, maturation, motility in after freezing. Similar studies, it can be improved by making changes in the cryopreservation method with the least amount of sperm DNA damage these changes will minimize the damage of frozen sperm DNA, better quality sperm can be obtained and the success of pregnancy and live birth rate.The study was submitted to the Biruni University Non-Interventional Ethics Committee and was approved by the ethics committee of 2018-12-9 and dated 29.01.2018. The study was carried out between September 2017 - April 2018 in patients applying for spermiogram analysis to Fertijin Women's Health and IVF Center. Among the patients who applied to IVF Center, standard semen analysis was performed according to WHO criteria in normozoospermia(n: 20) and OAT groups (n: 20). Sperm samples were washed using the in gradient, technique as a sperm washing method and were thawed with three different solutions as the cryoprotectant in the wash thecnique. They were thawed after 24 hours before freezing. Then all samples were evaluated with acridine orange and acidic alanine blue painting procedure on about sperm maturation and DNA fragmentation. According to this study, there is no significant difference between in sperm motility, DNA fragmentation and sperm maturation in these sperm samples after thawing or frozen with different cryopreservation solutions. Key words : Acridin orange, annilin blue, DNA fragmantation, sperm freezing, sperm maturation