Fikosiyaninin üretimi, izolasyonu, kısmi saflaştırılması ve bazı antioksidan-sitotoksik özelliklerinin araştırılması

In this thesis, in mixotrophic and heterotrophic cultivations to optimize the production conditions to obtain maximum phycocyanin (C-PC) from Arthrospira platensis-M2; carbon (crude/technical glycerol) and nitrogen (urea/sodium nitrate) sources were examined. C-PC optimum production conditions were determined on the 15th day in mixotrophic cultivation (1mM crude glycerol) as 3.75±0.12mg/g. Bioactive components like total protein, carbohydrate, lipid and carotenoid, chlorophyll-a/b, total phenolic, flavonoid and tannin, lipid peroxidation, pyruvate, and proline were also examined under optimum conditions. Then, to optimize C-PC extraction, wet, lyophilized, oven-dried, and frozen samples were mixed with ultrapure water/buffer at 4-different concentrations; and mixer mill, freeze-thaw, homogenization, and ultrasound-assisted extraction (UAE) were applied. C-PC extraction conditions were determined as 30 minutes at 80kHz in the simple and environmentally/friendly UAE of a 5 percent ultrapure water sample. Subsequently, ammonium sulfate (AS), organic solvent, and polyethylene glycol precipitation methods were applied. The best result was determined as AS precipitation at 50 percent saturation. After the sample was applied to DEAE-Sepharose column chromatography, the best fractions collected were combined and passed through the ultrafiltrate. Thus, C-PC with 2.3 reagent purity was obtained. Then, antioxidant (DPPH., HO., NO., ABTS.+, O2.-, total reducing power, FRAP, and metal chelation) and cytotoxic (OE-33 and HeLa cancer cell lines) properties were determined. Additionally, UV-Vis spectrum, FT-IR, and TGA characterizations of C-PC were performed. To the results, it appears that partially purified C-PC, with its economical production in the presence of crude glycerol, can be considered a value-added bioactive product with potential for use in various industrial fields.