A new photosensitive substance for photodynamic antimicrobial therapy

In photodynamic therapy (FDT), which is accepted as a promising treatment in the treatment of various diseases, including cancer and infectious diseases, it is a treatment that uses light to activate an agent that is photosensitive in the presence of oxygen and causes cell death with the formation of reactive radicals. Photosensitive material, which is one of the main components in photodynamic therapy, is one of the important parameters. Novel compounds are required and are under investigation to improve the effective use of photodynamic therapy. Possible applications and combinations of photosensitive substances synthesized in previous studies for photodynamic therapy provide significant guidance. In line with this information, the aim of the thesis is to synthesize photosensitive substances that can be used to strengthen the advantages and effects of photodynamic therapy in the application areas. Within the scope of this thesis, a new boron 2-(2'-pyridyl) imidazole (BOPIM) derivatization is used to synthesize a photosensitive substance that can be used effectively for photodynamic therapy. The BOPIM complex is a class that does not aggregate and emits fluorescence with a large stokes shift. Thanks to these advantageous properties, the photophysical properties and singlet oxygen generation capacity of this compound, which was synthesized as a result of proper derivatization, were investigated. 1,3-diphenylisobenzofuran (DPBF) was used as a singlet oxygen trap molecule. The decrease in absorbance of singlet oxygen DPBF formed as a result of the stimulation of the target molecule, BOPIM, was monitored. In addition, antimicrobial experimental studies were carried out. As a result, it was determined that the BOPIM molecule synthesized in the experimental studies was examined and it was determined that this structure produced singlet oxygen significantly and did not cause toxic effects in the dark environment, and its usability in photodynamic therapy was demonstrated. In order to elucidate the structure of the synthesized compound, measurements were made using 1H-NMR, 13C-NMR and MS techniques.